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Course 3: Flow cytometry in the study of biomarkers: basic notions and practical application

Topics covered

Morning shift: concepts and foundations of flow cytometry in scientific research, basics of immunology, principles of cytometry (lasers, optics, fluorides), fluorochrome and compensation. General applications of cytometry, such as distinction of cellular populations, immunophenotyping, evaluation of DNA content and biomarkers, functional tests, cytotoxicity, feasibility, cycle and cell proliferation

Afternoon shift: Practical development with probes/antibody marking, acquisition and analysis. Equipment sectage, channel compensation and data analysis using the Flowjo® program